PA007187: In Vivo Grade Recombinant Chimeric Human IgG1 kappa Isotype Control Antibody

The in vivo grade recombinant chimeric human IgG1 isotype control antibody was produced in CHO cells.
Applications: an isotype-matched negative control for human IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007188: In Vivo Grade Recombinant Chimeric Human IgG1-D265A kappa Isotype Control Antibody

The in vivo grade recombinant chimeric human IgG1-D265A kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 D265A kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007189: In Vivo Grade Recombinant Chimeric Human IgG1-N297A kappa Isotype Control Antibody

The in vivo grade recombinant chimeric human IgG1-N297A kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 N297A kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007190: In Vivo Grade Recombinant Chimeric Human IgG1-L234A L235A P329G (hIgG1 LALAPG) kappa Isotype Control Antibody

The in vivo grade recombinant chimeric human IgG1-L234A L235A P329G (hIgG1 LALAPG, Fc silenced) kappa isotype control mutant was produced in CHO cells.
Applications: an isotype-matched negative control for human IgG1 LALAPG kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007191: In Vivo Grade Recombinant Chimeric Human IgG1-D265A N297A (hIgG1 DANA) kappa Isotype Control Antibody

The in vivo grade recombinant chimeric human IgG1-D265A N297A (hIgG1 DANA) kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 DANA kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007192: In Vivo Grade Recombinant Chimeric Human IgG1-S267E L328F (hIgG1 SELF) kappa Isotype Control Antibody

The in vivo grade recombinant chimeric human IgG1-S267E L328F (hIgG1 SELF, Fc activated) kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 SELF kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007193: In vivo Grade Recombinant Chimeric Human IgG2 Isotype Control Antibody

Source: CHO cell-derived.
Applications: an isotype-matched negative control for human IgG2 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007194: In Vivo Grade Recombinant Chimeric Human IgG4-S228P Isotype Control Antibody (Chimeric hIgG4 S228P Isotype Control)

The recombinant chimeric human IgG4-S228P isotype control antibody (chimeric hIgG4 S228P isotype control) was produced in CHO cells.
Applications: an isotype-matched negative control for human IgG4 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007195: In Vivo Grade Recombinant Chimeric Human IgG4-S228P L235E P329G Isotype Control Antibody (Chimeric hIgG4 SPLEPG Isotype Control Mutant)

The recombinant chimeric human IgG4-S228P L235E P329G isotype control antibody (chimeric hIgG4 SPLEPG isotype control mutant, Fc silenced) was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG4 SPLEPG kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007157.m1: In vivo Grade Recombinant Anti-Human HER2/ErbB-2/c-neu Mouse IgG1 Monoclonal Antibody (muMAb 4D5)

Recombinant mouse IgG1 monoclonal antibody.
Specificity/Sensitivity: HER2 / ErbB-2 / c-neu.
Reactivity: Human. No cross reactivity to mouse, rat.
Applications: ELISA, neutralization, functional assays such as bioanalytical PK and ADA assays, and those assays for studying biological pathways affected by HER2 / ErbB-2 / c-neu.
Isotype: Mouse IgG1 kappa.
Clone: 4D5 (muMAb).

Syd Labs has extensive experience with making DNA constructs for Fc fusion protein production, expressing and purifying a number of Fc fusion proteins from microgram to kilogram scales in mammalian cells, such as CHO and HEK 293 cells. With improved DNA construction system and fully optimized secretion signal and linker sequences, Syd Labs can efficiently complete a project from DNA construction and transient production of Fc fusion proteins in CHO and HEK 293 cells, to Fc fusion protein purification. N-term or C-term of Fc fusions of peptides and proteins in secreted form and multiple Fc backbone are available upon request.

BS050B:
Synthesis of the gene encoding the Fc fusion protein;
Molecular construction of the recombinant Fc fusion protein;
0.04 Liter transient production of the recombinant Fc fusion protein in CHO or HEK293 cells;
Protein A purification.

Our FcRγ binding assay and FcRn binding assay services include: his-tagged receptors are immobilized onto anti-His biosensors; the loaded biosensors are dipped into a dilution series of the antibody; the on- (Kon) and off-rates (Koff) are measured and the KD value is calculated.

EK000095-HUFC: ELISA kit for human Fc protein

The human Fc ELISA complete kit contains the same essential components as the human Fc ELISA reagent kit (EK000095-20310).
Application: for the quantitative determination of human Fc proteins in cell culture supernatants and serum.
This assay is specific for human Fc, and does not cross react with mouse Fc.

EK000095-20310: ELISA reagent kit for human Fc protein

The human Fc ELISA Reagent Kit contains the same essential components as human Fc ELISA Complete Kit (EK000095-HUFC).
Application: for the quantitative determination of human Fc proteins in cell culture supernatants and serum.
This assay is specific for human Fc, and does not cross react with mouse Fc.

BP4091: In Vivo Grade Recombinant Human IgG1 Fc protein (hIgG1 Fc)

The recombinant human IgG1 Fc protein was produced in mammalian cells and purified by protein A.
Purity: >95% by SDS-PAGE under reducing conditions
Endotoxin: Less than 1 EU/mg of protein as determined by LAL method.

Recombinant human IgG1 Fc protein mutants available including hIgG1 Fc C103S; hIgG1 Fc D265A; hIgG1 Fc R214K; hIgG1 Fc R214K/N297A; hIgG1 Fc L234A/L235A; hIgG1 Fc L234F/L235E/P331S; hIgG1 Fc L235A/G237A. Recombinant human IgG1 isotype control antibody and mutants are available. Biotinylated hIgG1 Fc, and hIgG1 Fc with Avi-, His-, and Flag-tags are also available.

PA007125: In Vivo Grade Recombinant Human IgG1 kappa Isotype Control Antibody

The in vivo grade recombinant human IgG1 isotype control antibody was produced in CHO cells.
Applications: an isotype-matched negative control for human IgG1 kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method. Endotoxin ≤0.05 EU/mg for PA007125.05, in vivo grade recombinant human IgG1 isotype control antibody, premium grade (endotoxin ≤0.05 EU/mg).

PA007134: In Vivo Grade Recombinant Human IgG1-L234A L235A P329G (hIgG1 LALAPG) kappa Isotype Control Antibody

The in vivo grade recombinant human IgG1-L234A L235A P329G (hIgG1 LALAPG, Fc silenced) kappa isotype control mutant was produced in CHO cells.
Applications: an isotype-matched negative control for human IgG1 LALAPG kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method. Endotoxin ≤0.05 EU/mg for PA007134.05, in vivo grade recombinant human IgG1-L234A L235A P329G (hIgG1 LALAPG) isotype control antibody, premium grade (endotoxin ≤0.05 EU/mg).

PA007133: In Vivo Grade Recombinant Human IgG1-N297A kappa Isotype Control Antibody

The in vivo grade recombinant human IgG1-N297A kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 N297A kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007132: In Vivo Grade Recombinant Human IgG1-D265A kappa Isotype Control Antibody

The in vivo grade recombinant human IgG1-D265A kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 D265A kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007139: In Vivo Grade Recombinant Human IgG1-D265A N297A (hIgG1 DANA) kappa Isotype Control Antibody

The in vivo grade recombinant human IgG1-D265A N297A (hIgG1 DANA) kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 DANA kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.

PA007140: In Vivo Grade Recombinant Human IgG1-S267E L328F (hIgG1 SELF) kappa Isotype Control Antibody

The in vivo grade recombinant human IgG1-S267E L328F (hIgG1 SELF, Fc activated) kappa isotype control mutant was produced in mammalian cells.
Applications: an isotype-matched negative control for human IgG1 SELF kappa antibodies used in ELISA, Western Blot (WB), Flow Cytometry (Flow), Immunoprecipitation (IP), Immunohistochemistry (Paraffin) (IHC (P)), Immunohistochemistry (Frozen) (IHC (F)), and in vivo animal model research.
Endotoxin Level: Less than 1 EU/mg of protein as determined by LAL method.